OrangerV1, France, Analysis, bibRecord, 000234

Successful cryopreservation of suspension cells by encapsulation-dehydration

Identifieur interne : 000234 ( France/Analysis ); précédent : 000233; suivant : 000235

Successful cryopreservation of suspension cells by encapsulation-dehydration

Auteurs : Y. Bachiri [France] ; C. Gazeau [France] ; J. Hansz [France] ; C. Morisset [France] ; J. Dereuddre [France]

Source :

Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1995-12-01.

RBID : ISTEX:CE6DC28C5C30887702926D43F0C4BA19D50C0D21

Abstract

Abstract: Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate beads. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.

Url:

https://api.istex.fr/document/CE6DC28C5C30887702926D43F0C4BA19D50C0D21/fulltext/pdf

DOI: 10.1007/BF00039951

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000D04
to stream Istex, to step Curation: 000D04
to stream Istex, to step Checkpoint: 000E56
to stream Main, to step Merge: 003817
to stream Main, to step Curation: 003550
to stream Main, to step Exploration: 003550
to stream France, to step Extraction: 000234

Links to Exploration step

ISTEX:CE6DC28C5C30887702926D43F0C4BA19D50C0D21

Le document en format XML

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<author><name sortKey="Dereuddre, J" sort="Dereuddre, J" uniqKey="Dereuddre J" first="J." last="Dereuddre">J. Dereuddre</name>
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<front><div type="abstract" xml:lang="en">Abstract: Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate beads. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.</div>
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{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     ISTEX:CE6DC28C5C30887702926D43F0C4BA19D50C0D21
   |texte=   Successful cryopreservation of suspension cells by encapsulation-dehydration
}}

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Successful cryopreservation of suspension cells by encapsulation-dehydration

Successful cryopreservation of suspension cells by encapsulation-dehydration

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri